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J Virol Methods. 2012 Dec;186(1-2):126-31. doi: 10.1016/j.jviromet.2012.07.002. Epub 2012 Aug 4.

Evaluation of anti-norovirus IgY from egg yolk of chickens immunized with norovirus P particles.

Author information

1
Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Zip code 510515, Guangzhou, Guangdong, China.

Abstract

Noroviruses (NoVs) are a leading cause of epidemic acute gastroenteritis affecting millions of people worldwide. Understanding of NoV remains limited due to the lack of a cell culture system and small animal models. Currently, there are no effective vaccines or antivirals against NoVs. In this study, an approach for large-scale production of anti-NoV antibodies for use as a potential treatment for NoV disease using passive immunization was evaluated. NoV-specific immunoglobulins (IgYs) were produced by immunizing chickens with NoV P particles. The birds continuously produced high titers of antibodies in their eggs for at least 3months, in which NoV-specific antibody levels reached 4.7-9.2mg/egg yolk. The egg yolk antibodies strongly reacted with NoV P particles by both ELISA and Western blot and blocked NoV virus-like particle (VLP) and P particle binding to the histo-blood group antigen (HBGA) receptors with a BT(50) of about 1:800. The blocking activity of the chicken IgY remained after an incubation at 70°C for 30min or treatments at pH 4-9 for 3h. These data suggested that chicken IgY could be a practical strategy for large-scale production of anti-NoV antibodies for potential use as passive immunization against NoV infection, as well as for diagnostic purposes.

PMID:
22867844
PMCID:
PMC3496071
DOI:
10.1016/j.jviromet.2012.07.002
[Indexed for MEDLINE]
Free PMC Article

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