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Nat Methods. 2012 Sep;9(9):907-9. doi: 10.1038/nmeth.2131. Epub 2012 Aug 5.

A high-throughput approach for measuring temporal changes in the interactome.

Author information

1
Department of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada.

Abstract

Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.

PMID:
22863883
PMCID:
PMC3954081
DOI:
10.1038/nmeth.2131
[Indexed for MEDLINE]
Free PMC Article

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