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Nat Methods. 2012 Sep;9(9):910-2. doi: 10.1038/nmeth.2133. Epub 2012 Aug 5.

Direct observation of mammalian cell growth and size regulation.

Author information

1
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.

PMID:
22863882
PMCID:
PMC3433595
DOI:
10.1038/nmeth.2133
[Indexed for MEDLINE]
Free PMC Article

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