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ACS Chem Neurosci. 2012 May 16;3(5):364-8. doi: 10.1021/cn300001w. Epub 2012 Jan 18.

Anandamide externally added to lipid vesicles containing trapped fatty acid amide hydrolase (FAAH) is readily hydrolyzed in a sterol-modulated fashion.

Author information

1
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, United States.

Abstract

We show that anandamide (AEA) externally added to model membrane vesicles containing trapped fatty acid amide hydrolyase (FAAH) can be readily hydrolyzed, demonstrating facile, rapid anandamide movement across the lipid bilayer. The rate of hydrolysis is significantly facilitated by cholesterol and coprostanol, but not by cholesterol sulfate. The effects of sterol upon hydrolysis by FAAH bound to the outer surface of the bilayer were much smaller, although they followed the same pattern. We propose the facilitation of hydrolysis is a combination of the effects of sterol on accessibility of membrane-inserted endocannabinoids to surface protein, and on the rate of endocannabinod transport across the membrane bilayer.

KEYWORDS:

Anandamide; FAAH; LUV; cholesterol; fatty acid amide hydrolase; liposome; transporter

PMID:
22860204
PMCID:
PMC3382455
DOI:
10.1021/cn300001w
[Indexed for MEDLINE]
Free PMC Article
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