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FASEB J. 2012 Nov;26(11):4576-83. doi: 10.1096/fj.12-207183. Epub 2012 Aug 2.

Dual reporter approaches for identification of Cre efficacy and astrocyte heterogeneity.

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Life and Medical Sciences Institute, Medical Faculty, University of Bonn, Bonn, Germany.


Gene inactivation reporters are powerful tools to circumvent limitations of the widely used Cre/loxP system of conditional mutagenesis. With new conditional transgenic mouse lines expressing the enhanced cyan fluorescent protein (ECFP) instead of connexin43 (Cx43) after Cre-mediated recombination, we demonstrate dual reporter approaches to simultaneously examine astrocyte subpopulations expressing different connexins, identify compensatory up-regulation within gene families, and quantify Cre-mediated deletion at the allelic level. Analysis of a newly generated Cx43 knock-in ECFP mouse revealed an unexpected heterogeneity of Cx43-expressing astrocytes across brain areas.

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