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Nucleic Acids Res. 2012 Oct;40(19):9876-86. doi: 10.1093/nar/gks691. Epub 2012 Jul 30.

A distant cis acting intronic element induces site-selective RNA editing.

Author information

1
Department of Molecular Biology and Functional Genomics, Stockholm University, Svante Arrheniusväg 20C, SE-10691, Stockholm, Sweden.

Abstract

Transcripts have been found to be site selectively edited from adenosine-to-inosine (A-to-I) in the mammalian brain, mostly in genes involved in neurotransmission. While A-to-I editing occurs at double-stranded structures, other structural requirements are largely unknown. We have investigated the requirements for editing at the I/M site in the Gabra-3 transcript of the GABA(A) receptor. We identify an evolutionarily conserved intronic duplex, 150 nt downstream of the exonic hairpin where the I/M site resides, which is required for its editing. This is the first time a distant RNA structure has been shown to be important for A-to-I editing. We demonstrate that the element also can induce editing in related but normally not edited RNA sequences. In human, thousands of genes are edited in duplexes formed by inverted repeats in non-coding regions. It is likely that numerous such duplexes can induce editing of coding regions throughout the transcriptome.

PMID:
22848101
PMCID:
PMC3479170
DOI:
10.1093/nar/gks691
[Indexed for MEDLINE]
Free PMC Article

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