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Bioinformatics. 2012 Oct 1;28(19):2532-3. Epub 2012 Jul 30.

easyRNASeq: a bioconductor package for processing RNA-Seq data.

Author information

1
Genome Biology Computational Support, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. delhomme@embl.de

Abstract

MOTIVATION:

RNA sequencing is becoming a standard for expression profiling experiments and many tools have been developed in the past few years to analyze RNA-Seq data. Numerous 'Bioconductor' packages are available for next-generation sequencing data loading in R, e.g. ShortRead and Rsamtools as well as to perform differential gene expression analyses, e.g. DESeq and edgeR. However, the processing tasks lying in between these require the precise interplay of many Bioconductor packages, e.g. Biostrings, IRanges or external solutions are to be sought.

RESULTS:

We developed 'easyRNASeq', an R package that simplifies the processing of RNA sequencing data, hiding the complex interplay of the required packages behind a single functionality.

AVAILABILITY:

The package is implemented in R (as of version 2.15) and is available from Bioconductor (as of version 2.10) at the URL: http://bioconductor.org/packages/release/bioc/html/easyRNASeq.html, where installation and usage instructions can be found.

CONTACT:

delhomme@embl.de.

PMID:
22847932
PMCID:
PMC3463124
DOI:
10.1093/bioinformatics/bts477
[Indexed for MEDLINE]
Free PMC Article

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