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J Struct Biol. 2012 Oct;180(1):254-8. doi: 10.1016/j.jsb.2012.07.009. Epub 2012 Jul 25.

Creating an infrastructure for high-throughput high-resolution cryogenic electron microscopy.

Author information

1
Department of Scientific Computing, 400 Dirac Science Library, Florida State University, Tallahassee, FL 32306, USA.

Abstract

New instrumentation for three-dimensional electron microscopy is facilitating an increase in the throughput of data collection and reconstruction. The increase in throughput creates bottlenecks in the workflow for storing and processing the image data. Here we describe the creation and quantify the throughput of a high-throughput infrastructure supporting collection of three-dimensional data collection.

PMID:
22842049
PMCID:
PMC3466351
DOI:
10.1016/j.jsb.2012.07.009
[Indexed for MEDLINE]
Free PMC Article

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