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BMC Res Notes. 2012 Jul 28;5:385. doi: 10.1186/1756-0500-5-385.

Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos.

Author information

1
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.

Abstract

BACKGROUND:

The 7S globulins are plant seed storage proteins that have been associated with the development of a number of human diseases, including peanut allergy. Immune reactivity to the wheat seed storage protein globulin-3 (Glo-3) has been associated with the development of the autoimmune disease type 1 diabetes in diabetes-prone rats and mice, as well as in a subset of human patients.

FINDINGS:

The present study characterized native wheat Glo-3 in salt-soluble wheat seed protein extracts. Glo-3-like peptides were observed primarily in the wheat embryo. Glo-3-like proteins varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting with anti-Glo-3A antibodies. Five major polypeptide spots were identified by mass spectrometry and N-terminal sequencing as belonging to the Glo-3 family.

CONCLUSIONS:

These results in combination with our previous findings have allowed for the development of a hypothetical model of the post-translational events contributing to the wheat 7S globulin profile in mature wheat kernels.

PMID:
22838494
PMCID:
PMC3434096
DOI:
10.1186/1756-0500-5-385
[Indexed for MEDLINE]
Free PMC Article
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