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Methods. 2012 Oct;58(2):171-87. doi: 10.1016/j.ymeth.2012.07.020. Epub 2012 Jul 23.

Bioinformatic analysis of barcoded cDNA libraries for small RNA profiling by next-generation sequencing.

Author information

1
Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10065, USA.

Abstract

The characterization of post-transcriptional gene regulation by small regulatory RNAs of 20-30 nt length, particularly miRNAs and piRNAs, has become a major focus of research in recent years. A prerequisite for the characterization of small RNAs is their identification and quantification across different developmental stages, normal and diseased tissues, as well as model cell lines. Here we present a step-by-step protocol for the bioinformatic analysis of barcoded cDNA libraries for small RNA profiling generated by Illumina sequencing, thereby facilitating miRNA and other small RNA profiling of large sample collections.

PMID:
22836126
PMCID:
PMC3597438
DOI:
10.1016/j.ymeth.2012.07.020
[Indexed for MEDLINE]
Free PMC Article

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