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Pathobiology. 2013;80(1):24-31. doi: 10.1159/000339430. Epub 2012 Jul 24.

Increased shedding of microvesicles from intimal smooth muscle cells in athero-prone areas of the human aorta: implications for understanding of the predisease stage.

Author information

1
Institute for Atherosclerosis Research, Russian Academy of Natural Sciences, Moscow, Russia. y.bobryshev@unsw.edu.au

Abstract

OBJECTIVE:

This study evaluated whether a change in the content of matrix microvesicles might occur at the preatherosclerotic stage.

METHODS:

Applying quantitative electron microscopic and immunohistochemical analyses, two areas of grossly normal segments of the thoracic aorta were compared: atherosclerosis-prone (AP) areas, situated at the dorsal aspect of the aorta along the rows of intercostal branch origins, and atherosclerosis-resistant (AR) areas, situated at the corresponding sites of the ventral aspect of the aorta.

RESULTS:

The electron microscopic analysis showed that there were 1.4 times more microvesicles in AP areas than AR areas (p = 0.019). It was found that matrix microvesicles originated as a result of blebbing and shedding of surface membranes of smooth muscle cells. A quantitative analysis of the expression of ADP-ribosylation factor 6 (ARF6), which is known to be involved in membrane trafficking and microvesicle formation, showed that ARF6 expression was 1.3 times higher in AP areas than that in AR areas (p = 0.006). There was a positive correlation between the content of matrix microparticles and the expression of ARF6 by intimal smooth muscle cells (r = 0.61; p < 0.0001).

CONCLUSION:

The present study supports the concept that alterations of the arterial intima occur at the predisease stage.

PMID:
22832241
DOI:
10.1159/000339430
[Indexed for MEDLINE]
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