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Methods Mol Biol. 2012;912:199-209. doi: 10.1007/978-1-61779-971-6_12.

Culture systems: single step.

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Obstetrics, Gynecology & Reproductive Biology, Brigham and Women's Hospital, Boston, MA, USA.


Culture media to support development of zygotes to the blastocyst stage is based either on a single medium or sequential (two-step) media. Single medium culture either with or without day 3 renewal is associated with simplified laboratory protocols and lower costs compared with sequential medium. There are currently insufficient clinical data to conclude that one system, either single or sequential, is superior to the other with regard to clinical performance. This chapter summarizes the rationale for use of a single medium, along with supporting animal and human data for culturing embryos in a single medium, renewed or not renewed.

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