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Biophys J. 2012 Jul 3;103(1):11-8. doi: 10.1016/j.bpj.2012.05.020.

Label-free imaging of membrane potential using membrane electromotility.

Author information

1
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. seungeun_oh@hms.harvard.edu

Abstract

Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.

PMID:
22828327
PMCID:
PMC3388206
DOI:
10.1016/j.bpj.2012.05.020
[Indexed for MEDLINE]
Free PMC Article

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