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Transgenic Res. 2013 Feb;22(1):195-200. doi: 10.1007/s11248-012-9635-x. Epub 2012 Jul 24.

Production of mouse pups from germline transmission-failed knockout chimeras.

Author information

1
Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, 565-0871, Osaka, Japan.

Abstract

Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.

PMID:
22826106
DOI:
10.1007/s11248-012-9635-x
[Indexed for MEDLINE]

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