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Mar Drugs. 2012 Jun;10(6):1192-202. doi: 10.3390/md10061192. Epub 2012 May 25.

Diversity of nonribosomal peptide synthetase genes in the microbial metagenomes of marine sponges.

Author information

1
Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main St W, Hamilton, ON L8S 4K1, Canada. elardos@univmail.cis.mcmaster.ca

Abstract

Genomic mining revealed one major nonribosomal peptide synthetase (NRPS) phylogenetic cluster in 12 marine sponge species, one ascidian, an actinobacterial isolate and seawater. Phylogenetic analysis predicts its taxonomic affiliation to the actinomycetes and hydroxy-phenyl-glycine as a likely substrate. Additionally, a phylogenetically distinct NRPS gene cluster was discovered in the microbial metagenome of the sponge Aplysina aerophoba, which shows highest similarities to NRPS genes that were previously assigned, by ways of single cell genomics, to a Chloroflexi sponge symbiont. Genomic mining studies such as the one presented here for NRPS genes, contribute to on-going efforts to characterize the genomic potential of sponge-associated microbiota for secondary metabolite biosynthesis.

KEYWORDS:

NRPS; Porifera; marine sponge; metagenomics; nonribosomal peptide synthetase

PMID:
22822366
PMCID:
PMC3397433
DOI:
10.3390/md10061192
[Indexed for MEDLINE]
Free PMC Article

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