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Nucleic Acids Res. 2012 Oct;40(18):8862-73. doi: 10.1093/nar/gks684. Epub 2012 Jul 20.

Computational identification of new structured cis-regulatory elements in the 3'-untranslated region of human protein coding genes.

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1
Department of Biochemistry and Genetics Otago, University of Otago, Dunedin 9054, New Zealand. sylvia.x.chen@gmail.com

Abstract

Messenger ribonucleic acids (RNAs) contain a large number of cis-regulatory RNA elements that function in many types of post-transcriptional regulation. These cis-regulatory elements are often characterized by conserved structures and/or sequences. Although some classes are well known, given the wide range of RNA-interacting proteins in eukaryotes, it is likely that many new classes of cis-regulatory elements are yet to be discovered. An approach to this is to use computational methods that have the advantage of analysing genomic data, particularly comparative data on a large scale. In this study, a set of structural discovery algorithms was applied followed by support vector machine (SVM) classification. We trained a new classification model (CisRNA-SVM) on a set of known structured cis-regulatory elements from 3'-untranslated regions (UTRs) and successfully distinguished these and groups of cis-regulatory elements not been strained on from control genomic and shuffled sequences. The new method outperformed previous methods in classification of cis-regulatory RNA elements. This model was then used to predict new elements from cross-species conserved regions of human 3'-UTRs. Clustering of these elements identified new classes of potential cis-regulatory elements. The model, training and testing sets and novel human predictions are available at: http://mRNA.otago.ac.nz/CisRNA-SVM.

PMID:
22821558
PMCID:
PMC3467077
DOI:
10.1093/nar/gks684
[Indexed for MEDLINE]
Free PMC Article
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