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Saudi Med J. 2012 Jul;33(7):716-21.

Helicobacter pylori virulence markers in gastroduodenal disorders. Detection of cytotoxin-associated gene A and vacuolating cytotoxin-associated gene A genes in Saudi patients.

Author information

1
Department of Pathology, College of Medicine, King Khalid University Hospital, King Saud University, PO Box 2925, Riyadh 11461, Kingdom of Saudi Arabia. alkhattaf2@hotmail.com

Abstract

OBJECTIVE:

To detect the presence of virulence markers cytotoxin-associated (cagA) and vacuolating cytotoxin-associated (vacA) genes in gastric biopsy specimens of patients with gastroduodenal disorders.

METHODS:

This study was conducted at the Department of Pathology, King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia between March 2008 and February 2009. A total of 118 gastric biopsy specimens from 81 males and 37 females (mean age: 55 +/- 18 years) with histological evidence for the presence of Helicobacter pylori (H. pylori) were included in the study. The H. pylori cagA and vacA genes were detected using polymerase chain reaction-enzyme-linked immunosorbent assay technique.

RESULTS:

Both H. pylori cagA and vacA genes were detected in 60 (51%) patients. Forty-one (35%) patients had active chronic gastritis, 22 (54%) harbored cagA, and 25 (61%) had vacA gene. Twenty-six (22%) patients had duodenal ulcer, 14 (54%) had cagA, and 15 (58%) had vacA genes. Eighteen (15%) patients had active acute gastritis, 8 (44%) carrying cagA gene, and 12 (67%) had vacA gene. The cagA and vacA genes co-existed in all the 17 (100%) patients with adenocarcinoma. These genes coexisted in 44% biopsies from active acute gastritis, and 46% each in duodenal ulcer and active chronic gastritis.

CONCLUSION:

The cagA and vacA genes as H. pylori virulence markers were detected in gastroduodenal disorders, and their remarkably high co-existence in adenocarcinoma prompt further investigations for evaluating H. pylori as a direct carcinogen.

PMID:
22821304
[Indexed for MEDLINE]

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