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Acta Biomater. 2012 Nov;8(11):4130-8. doi: 10.1016/j.actbio.2012.07.011. Epub 2012 Jul 20.

Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient.

Author information

1
Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.

Abstract

Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD.

PMID:
22820310
PMCID:
PMC3576574
DOI:
10.1016/j.actbio.2012.07.011
[Indexed for MEDLINE]
Free PMC Article
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