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FEBS Lett. 2012 Sep 21;586(19):3317-23. doi: 10.1016/j.febslet.2012.07.008. Epub 2012 Jul 20.

N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells.

Author information

1
Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO 65212, USA.

Abstract

Here we show that tyrosine phosphorylation of caveolin-2 (Cav-2) negatively regulates the anti-proliferative function of transforming growth factor beta (TGF-beta) in endothelial cells. In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. Conversely, although less effective than wild-type, re-expression of S23/36A-Cav-2 reduced the effect of TGF-beta compared to empty vector. This differential effect of tyrosine and serine phosphorylation mutants of Cav-2 correlated with TGF-beta-induced Smad3 phosphorylation and transcriptional activation of plasminogen activator inhibitor-1. Thus tyrosine-phosphorylated Cav-2 counteracts anti-proliferative effect of TGF-beta in endothelial cells.

PMID:
22819829
PMCID:
PMC3586282
DOI:
10.1016/j.febslet.2012.07.008
[Indexed for MEDLINE]
Free PMC Article

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