(A) Diagram schematically depicts the 28 sub-models as colored words – grouped by category as metabolic (orange), RNA (green), protein (blue), and DNA (red) – in the context of a single M. genitalium cell with its characteristic flask-like shape. Sub-models are connected through common metabolites, RNA, protein, and the chromosome which are depicted as orange, green, blue, and red colored arrows, respectively.
(B) The model integrates cellular function sub-models through 16 cell state variables. First, simulations are randomly initialized to the beginning of the cell cycle (left grey arrow). Next, for each 1 s time step (dark black arrows) the sub-models retrieve the current values of the cellular variables, calculate their contributions to the temporal evolution of the cell variables, and update the values of the cellular variables. This is repeated thousands of times over the course of each simulation. For clarity, cell functions and variables are grouped into 5 physiologic categories: DNA (red), RNA (green), protein (blue), metabolite (orange), and other (black). Colored lines between the variables and sub-models indicate the cell variables predicted by each sub-model. The number of genes associated with each sub-model is indicated in parentheses. Finally, simulations are terminated upon cell division when the septum diameter equals zero (right grey arrow).

(A) Growth of three cultures (dilutions indicated by shade of blue) and a blank control measured by OD550 of the pH indicator phenol red. The doubling time, τ, was calculated using the equation at the top left from the additional time required by more dilute cultures to reach the same OD550 (black lines).
(B) Predicted growth dynamics of one life cycle of a population of 64 in silico cells (randomly chosen from the total simulation set). Median cell is highlighted in red. Distribution of cell cycle lengths is shown at bottom.
(C) Comparison of the predicted and experimentally observed () cellular chemical compositions. Red bars indicate model s.d.; Morowitz et al. did not report s.d.
(D) Temporal dynamics of the total cell mass and four cell mass fractions of a representative in silico cell. Mass fractions are normalized to their initial values.
(E) Average predicted metabolic fluxes (see for metabolite and reaction labels). Arrow brightness indicates flux magnitude. The ratios of the GpsA and TalA fluxes to the Glk flux are indicated in orange boxes and are comparable to experimental data ().
(F) Ratios of observed (; ) and average predicted concentrations of 39 metabolites.
(G) Temporal dynamics of cytadherence high molecular weight protein 2 (HMW2, MG218) mRNA and protein expression of one in silico cell. Red dashed lines indicate the direct link between mRNA synthesis and subsequent bursts in protein synthesis.
(H) HMW2 mRNA and protein copy number distribution of an unsynchronized population of 128 in silico cells. Histograms indicate the marginal distributions of the copy numbers of mRNA (top) and protein (right). Red lines indicate log-normal regressions of these marginal distributions. The absence of correlation between the copy numbers of mRNA and protein and the shapes of the marginal distributions are consistent with recent single-cell measurements by .
See also , and and .

(A) Average density of all DNA-bound proteins and of the replication initiation protein DnaA and DNA and RNA polymerase of a population of 128 in silico cells. Top magnification indicates the average density of DnaA at several sites near the oriC; DnaA forms a large multimeric complex at the sites indicated with asterisks, recruiting DNA polymerase to the oriC to initiate replication. Bottom left label indicates the location of the highly expressed rRNA genes.
(B and C) Percentage of the chromosome that is predicted to have been bound (B), and the number of genes that are predicted to have been expressed (C) as functions of time. SMC is an abbreviated name for the name of the chromosome partition protein (MG298).
(D) DNA-binding and dissociation dynamics of the oriC DnaA complex (red) and of RNA (blue) and DNA (green) polymerases for one in silico cell. The oriC DnaA complex recruits DNA polymerase to the oriC to initiate replication, which in turn dissolves the oriC DnaA complex. RNA polymerase traces (blue line segments) indicate individual transcription events. The height, length, and slope of each trace represent the transcript length, transcription duration, and transcript elongation rate, respectively. Inset highlights several predicted collisions between DNA and RNA polymerases leading to the displacement of RNA polymerases and incomplete transcripts.

(A) Distributions of the duration of three cell cycle phases, as well as that of the total cell cycle length, across 128 simulations.
(B) Dynamics of macromolecule abundance in a selected cell simulation: top, the size of the DnaA complex assembling at the OriC (in monomers of DnaA); middle, the copy number of the chromosome; and bottom, the cytosolic dNTP concentration. The quantities of these macromolecules correlate strongly with the timing of key cell cycle stages.
(C) Correlation between the initial cellular DnaA content and the duration of the replication initiation cell cycle stage across the same 128 in silico cells depicted in (A).
(D) Correlation between the dNTP concentrations (both at the beginning of the cell cycle and at the beginning of replication) and the duration of replication across the same 128 in silico cells depicted in (A).
(E) Correlation between the duration of replication initiation and replication across the same 128 in silico cells depicted in (A).

(A) Intracellular concentrations of the energy carriers ATP, GTP, FAD(H₂), NAD(H), and NADP(H) of one in silico cell.
(B) Comparison of the cell cycle length and total ATP and GTP usage of 128 in silico cells.
(C) ATP (blue) and GTP (green) usage of 15 cellular processes throughout the life cycle of one in silico cell. The pie charts at right denote the percentage of ATP and GTP usage (red) as a fraction of total usage. (D) Average distribution of ATP and GTP usage among all modeled cellular processes in a population of 128 in silico cells. In total, the modeled processes account for only 44.3% of the amount of energy that has been experimentally observed to be produced during cellular growth.

(A) Comparison of predicted and observed () gene essentiality. Model predictions are based on at least five simulations of each single-gene disruption strain; see for details.
(B) Single-gene disruption strains were grouped into phenotypic classes (columns) according to their capacity to grow, synthesize protein, RNA, and DNA, and divide (indicated by septum length). Each column depicts the temporal dynamics of one representative in silico cell of each essential disruption strain class. Disruption strains of non-essential genes are not shown. Dynamics significantly different from wild type are highlighted in red. The identity of the representative cell and the number of disruption strains in each category is indicated in parenthesis.
(C and D) Degradation and dilution of N-terminal protein content (C) of methionine aminopeptidase (map, MG172) disrupted cells causes reduced growth (D). Blue and black lines indicate the map disruption and wild type strains, respectively. Horizontal bars indicate s.d.
See also for the distribution of simulated growth rates.

(A) Comparison of measured and predicted growth rates for wild type and 12 single-gene disrupted strains. Model predictions that fall within the shaded region were considered consistent with experimental observations; the region has a width of four times the standard deviation of the wild type strain growth measurement. Error bars represent s.d.
(B) Growth curves for the wild type and lpdA gene disruption strains and blank; similar to .
(C) Expectation values determined by performing a pBLAST search of the M. genitalium genome with the LpdA sequence as a query. The asterisk and colored bar indicate a significant match (E < 10⁻⁶).
(D) Detail of the M. genitalium genome. The pyruvate dehydrogenase complex genes are indicated by the top bracket, and transcription units identified in M. pneumoniae () are indicated by arrows. The transcription unit including nox is highlighted in color.
(E) Allowing Nox to partially replace LpdA in pyruvate dehydrogenase reconciles model predictions and experimental observations. The blue and red lines represent the predicted wild type and ΔlpdA strain growth rates as a function of the Nox-pyruvate dehydrogenase k_cat. The pink box indicates the k_cat at which the model predictions are consistent with both the wild type and ΔlpdA strain experimentally measured growth rates.
(F and G) Diagnosing the discrepancy between predictions and experiment for the thyA (F) and deoD (G) gene disruption strains. Some of the functionalities of ThyA and DeoD can be replaced by the enzymes Tdk and Pdp, respectively. The predicted growth rates of the wild type and gene disruption strains depend on the k_cat of these enzymes. The green region highlights the range of k_cat values consistent with the measured growth rates of both the wild type and gene disruption strain.
(H) Newly predicted k_cat values are similar to values that were measured in closely related organisms. Measured values of k_cat for Tdk (top) and Pdp (bottom) are shown; green arrow indicates the initial and revised k_cat values. The nearest M. genitalium relative is highlighted in green.
(I) Model-based biological discovery. Comparison of model predictions to experimental measurements identified gene disruption strains of particular interest, including the lpdA, deoD and thyA disruption strains. Further investigation – using a combination of experiments, modeling and/or informatics – led to new and more consistent measurements and predictions. Most importantly, the higher consistency reflected novel insights into M. genitalium biology. The arrows (red for lpdA, green for deoD and thyA) indicate the shift from lower to higher consistency between model and experiment, and each arrow is annotated with the new biological insight and the supporting evidence in parentheses. The overall graph format is the same as Figure 7A.