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Clin Exp Reprod Med. 2012 Jun;39(2):73-80. doi: 10.5653/cerm.2012.39.2.73. Epub 2012 Jun 30.

Effects of hypoxia inducible factors-1α on autophagy and invasion of trophoblasts.

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1
Department of Biomedical Science, CHA University, Seoul, Korea.

Abstract

OBJECTIVE:

This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-1α on the cell death, autophagy, and invasion of trophoblasts.

METHODS:

To understand the effect of HIF-1α, we inhibited HIF-1α using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-1α. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy.

RESULTS:

There was no difference in cell death through the inhibition of HIF-1α expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-1α expression by siRNA. These experimental results mean that HIF-1α genes regulate the invasive ability of trophoblasts by increasing autophagy.

CONCLUSION:

This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-1α and autophagy.

KEYWORDS:

Hypoxia; Invasiveness; Placenta; Programmed cell death type II; Trophoblasts

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