Format

Send to

Choose Destination
See comment in PubMed Commons below
Chembiochem. 2012 Aug 13;13(12):1717-24. doi: 10.1002/cbic.201200226. Epub 2012 Jul 17.

Patterned self-assembled monolayers: efficient, chemically defined tools for cell biology.

Author information

1
Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Engineering Centers Building, Madison, WI 53706, USA.

Abstract

Self-assembled monolayers (SAMs) of alkanethiolates on gold can be used to carefully probe immobilized biomolecule interactions with cell-surface receptors. However, due to a lack of experimental throughput associated with labor-intensive production, specialized fabrication apparatus, and other practical challenges, alkanethiolate SAMs have not had widespread use by biological researchers. In this Minireview, we investigate a range of techniques that could enhance the throughput of SAM-based approaches by patterning substrates with arrays of different conditions. Here we highlight microfluidic, photochemical, localized removal, and backfilling techniques to locally pattern SAM substrates with biomolecules and also describe how these approaches have been applied in SAM-based screening systems. Furthermore we provide perspectives on several crucial barriers that need to be overcome to enable widespread use of SAM chemistry in biological applications.

PMID:
22807236
PMCID:
PMC3995495
DOI:
10.1002/cbic.201200226
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center