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Wei Sheng Wu Xue Bao. 2012 May 4;52(5):645-53.

[Microbial community in nitrogen cycle of aquaculture water of the Pearl River Delta].

[Article in Chinese]

Author information

1
School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, China. alancai2011@163.com

Abstract

OBJECTIVE:

In order to study the characteristic of nitrogen transport, the community structure and diversity of related microorganisms in aquaculture water of the Pearl River Delta.

METHODS:

We established an artificial aquaculture ecosystem to study the microbial community of 15N-stable isotope probing (15N-SIP) labeled nitrogen transport microorganisms. The 15N-labeled DNA was separated by CsCl-ethidium bromide density gradient centrifugation, and was used to construct 16S rRNA gene clone libraries of bacteria and archaea.

RESULTS:

Phylogenetic analysis shows that 19 Operational Taxonomic Units (OTUs) from bacterial library were clustered in Proteobacteria and Planctomycetes. Proteobacteria (99.2%) was the dominant group, mainly consisted of Comamonas (15.7%), Nitrosomonas (12.4%), Enterobacteriaceae (11.5%) and Nitrobacter (11.5%). From archaeal library 9 OTUs were divided into 3 phyla: Thaumarchaeota, Crenarchaeota and Euryarchaeota.

CONCLUSION:

We successfully elucidated the microbial community of nitrogen transport microorganisms in aquaculture water of Pearl River Delta by using 15N-SIP. The data of the community will provide essential information for isolating nitrogen degrading microorganism, and provide scientific basis for creating a healthy aquaculture environment.

PMID:
22803351
[Indexed for MEDLINE]

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