Proliferation of Walker 256 carcinosarcoma cells: effect of whole-body hyperthermia and antitumor agents

E L Lushnikova; E V Ovsyanko; L M Nepomnyashchikh; A V Efremov; D V Morozov

doi:10.1007/s10517-011-1475-9

Proliferation of Walker 256 carcinosarcoma cells: effect of whole-body hyperthermia and antitumor agents

Bull Exp Biol Med. 2011 Nov;152(1):146-52. doi: 10.1007/s10517-011-1475-9.

[Article in English, Russian]

Authors

E L Lushnikova¹, E V Ovsyanko, L M Nepomnyashchikh, A V Efremov, D V Morozov

Affiliation

¹ Research Institute of Regional Pathology and Pathomorphology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. pathol@soramn.ru

PMID: 22803062
DOI: 10.1007/s10517-011-1475-9

Abstract

We studied the main biological characteristics of spontaneous growth of Walker 256 carcinosarcoma and under conditions of antitumor therapy. A combination of whole-body hyperthermia and cytostatic treatment (cyclophosphamide and melatonin) produced maximum suppression of the tumor growth, inhibition of mitotic activity of tumor cells, and stimulation of their necrotic and apoptotic death. The maximum decrease in mitotic activity of tumor cells was observed after combined exposure to whole-body hyperthermia and both cytostatic preparations; enhancement of apoptotic cell death and the decrease in the tumor node weight were also most pronounced under these conditions and practically no body weight loss was recorded in this case.

MeSH terms

Animals
Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
Apoptosis
Carcinoma 256, Walker / pathology*
Carcinoma 256, Walker / therapy*
Cell Line, Tumor
Cell Proliferation*
Combined Modality Therapy
Cyclophosphamide / administration & dosage
Drug Screening Assays, Antitumor
Hyperthermia, Induced*
Melatonin / administration & dosage
Neoplasm Transplantation
Rats
Rats, Wistar
Tumor Burden / drug effects

Substances

Cyclophosphamide
Melatonin