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Sci Rep. 2012;2:509. doi: 10.1038/srep00509. Epub 2012 Jul 13.

Nucleoprotein filament formation is the structural basis for bacterial protein H-NS gene silencing.

Author information

1
NUS Graduate school For Integrative Sciences and Engineering, Singapore 119077.

Abstract

H-NS is an abundant nucleoid-associated protein in bacteria that globally silences genes, including horizontally-acquired genes related to pathogenesis. Although it has been shown that H-NS has multiple modes of DNA-binding, which mode is employed in gene silencing is still unclear. Here, we report that in H-NS mutants that are unable to silence genes, are unable to form a rigid H-NS nucleoprotein filament. These results indicate that the H-NS nucleoprotein filament is crucial for its gene silencing function, and serves as the fundamental structural basis for gene silencing by H-NS and likely other H-NS-like bacterial proteins.

PMID:
22798986
PMCID:
PMC3396134
DOI:
10.1038/srep00509
[Indexed for MEDLINE]
Free PMC Article

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