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J Cell Sci. 2012 Oct 15;125(Pt 20):4770-9. doi: 10.1242/jcs.104059. Epub 2012 Jul 13.

The centriolar satellite protein Cep131 is important for genome stability.

Author information

1
Genome Stability Group, CR-UK/YCR Sheffield Cancer Research Centre, YCR Institute for Cancer Studies, Department of Oncology, University of Sheffield Medical School, UK.

Abstract

The centrosome acts as a centre for microtubule organisation and plays crucial roles in cell polarity, migration, growth and division. Cep131 has recently been described as a basal body component essential for cilium formation, but its function in non-ciliogenic cells is unknown. We identified human Cep131 (also known as AZI1) in a screen for regulators of genome stability. We show that centrosomal localisation of Cep131 is cell-cycle-regulated and requires both an intact microtubule network and a functional dynein-dynactin transport system. Cep131 is recruited to centriolar satellites by PCM1, and localised to the centriolar core region by both pericentrin and Cep290. Depletion of Cep131 results in a reduction in proliferation rate, centriole amplification, an increased frequency of multipolar mitosis, chromosomal instability and an increase in post-mitotic DNA damage. These data therefore highlight the importance of human Cep131 for maintaining genomic integrity.

PMID:
22797915
DOI:
10.1242/jcs.104059
[Indexed for MEDLINE]
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