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Obes Facts. 2012;5(3):440-51. doi: 10.1159/000341137. Epub 2012 Jun 30.

Changes in gene expression profile in human subcutaneous adipose tissue during significant weight loss.

Author information

1
Service of Endocrinology, Diabetes and Metabolism, University Hospital CHUV, Lausanne, Switzerland.

Abstract

OBJECTIVE:

To analyze the expression of peroxisome proliferator-activated receptor-γ1 and 2 (PPARγ1 and 2), 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), and leptin in adipose tissue (AT) of obese women during weight loss following Roux-en-Y gastric bypass (RYGB) and to compare these levels with those obtained in AT of nonobese subjects.

METHODS:

Gene expression was determined by real-time RT-PCR prior to surgery and at 3, 6, and 12 months after RYGB.

RESULTS:

All obese patients lost weight, reaching a mean BMI of 29.3 ± 1.0 kg/m(2) at 1 year after surgery (-33.9 ± 1.5% of their initial body weight). In obese subjects leptin and 11βHSD1 were over-expressed, whereas PPARγ1 was expressed at lower levels compared to controls. After surgery, leptin and 11βHSD1 gene expression decreased, whereas PPARγ1 expression increased. At 12 months after RYGB, these 3 genes had reached levels similar to the controls. In contrast, PPARγ2 gene expression was not different between groups and types of tissue and remained unchanged during weight loss. We found a positive correlation between BMI and levels of gene expression of leptin and 11βHSD1.

CONCLUSION:

Gene expression of leptin, PPARγ1, and 11βHSD1 in AT is modified in human obesity. This default is completely corrected by RYGB.

PMID:
22797372
DOI:
10.1159/000341137
[Indexed for MEDLINE]
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