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Biosci Biotechnol Biochem. 2012;76(6):1163-8. Epub 2012 Jun 7.

Important role of β1-integrin in fucoidan-induced apoptosis via caspase-8 activation.

Author information

1
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. yamasakiy@cc.miyazaki-u.ac.jp

Abstract

Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β1-integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β1-integrin-caspase-8 complex. These data indicate that β1-integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β1-integrin intracellular domain, cleaved it into the activated protein by direct combination with β1-integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.

PMID:
22790940
DOI:
10.1271/bbb.111001
[Indexed for MEDLINE]
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