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J Vet Diagn Invest. 2012 Sep;24(5):964-7. doi: 10.1177/1040638712452112. Epub 2012 Jul 11.

Evaluation of cefoxitin disk diffusion breakpoint for detection of methicillin resistance in Staphylococcus pseudintermedius isolates from dogs.

Author information

1
Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USA. bemis@utk.edu

Abstract

Cefoxitin disk diffusion susceptibility testing is a recommended screening method for the detection of methicillin resistance in human isolates of Staphylococcus aureus and coagulase-negative staphylococci. A retrospective analysis of 1,146 clinical isolates of Staphylococcus pseudintermedius from dogs was conducted to determine if screening by the cefoxitin disk method can be similarly useful with S. pseudintermedius. The distribution of cefoxitin growth inhibition zone diameters within this collection was bimodal and correlated well with the results of methicillin resistance gene (mecA) detection by polymerase chain reaction. Of the isolates, 5% had discordant results and, when retested, 84% of these were in agreement. While a greater diversity of isolates and interlaboratory comparisons must be tested, the current study suggests that an epidemiological breakpoint (of approximately ≤ 30 mm = resistant; ≥ 31 = susceptible) can be established to predict methicillin resistance in S. pseudintermedius.

PMID:
22786971
DOI:
10.1177/1040638712452112
[Indexed for MEDLINE]

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