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J Am Chem Soc. 2012 Jul 25;134(29):11974-6. doi: 10.1021/ja305734x. Epub 2012 Jul 13.

An intramolecular G-quadruplex structure is required for binding of telomeric repeat-containing RNA to the telomeric protein TRF2.

Author information

1
Cancer Research UK Cambridge Research Institute, Robinson Way, Cambridge CB2 0RE, UK.

Abstract

Telomeric repeat-containing RNA (TERRA) is important for telomere regulation, but the structural basis for how TERRA localizes to chromosome ends is unknown. Here we report on studies exploring whether the TERRA G-quadruplex structure is critical for binding to telomeres. We demonstrate that the telomeric protein TRF2 binds TERRA via interactions that necessitate the formation of a G-quadruplex structure rather than the TERRA sequence per se. We also show that TRF2 simultaneously binds TERRA and telomeric duplex or G-quadruplex DNA. These observations suggest that the TERRA G-quadruplex is a key feature of telomere organization.

PMID:
22780456
PMCID:
PMC3528108
DOI:
10.1021/ja305734x
[Indexed for MEDLINE]
Free PMC Article

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