Format

Send to

Choose Destination
See comment in PubMed Commons below
Science. 2012 Jul 27;337(6093):470-3. doi: 10.1126/science.1219861. Epub 2012 Jul 8.

Absence of detectable arsenate in DNA from arsenate-grown GFAJ-1 cells.

Author information

1
Lewis Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.

Abstract

A strain of Halomonas bacteria, GFAJ-1, has been claimed to be able to use arsenate as a nutrient when phosphate is limiting and to specifically incorporate arsenic into its DNA in place of phosphorus. However, we have found that arsenate does not contribute to growth of GFAJ-1 when phosphate is limiting and that DNA purified from cells grown with limiting phosphate and abundant arsenate does not exhibit the spontaneous hydrolysis expected of arsenate ester bonds. Furthermore, mass spectrometry showed that this DNA contains only trace amounts of free arsenate and no detectable covalently bound arsenate.

PMID:
22773140
PMCID:
PMC3845625
DOI:
10.1126/science.1219861
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center