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Artif DNA PNA XNA. 2012 Apr-Jun;3(2):63-72. doi: 10.4161/adna.20603. Epub 2012 Apr 1.

Selective recognition of DNA from olive leaves and olive oil by PNA and modified-PNA microarrays.

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1
Dipartimento di Chimica Organica e Industriale, Università di Parma, Parma, Italy.

Abstract

PNA probes for the specific detection of DNA from olive oil samples by microarray technology were developed. The presence of as low as 5% refined hazelnut (Corylus avellana) oil in extra-virgin olive oil (Olea europaea L.) could be detected by using a PNA microarray. A set of two single nucleotide polymorphisms (SNPs) from the Actin gene of Olive was chosen as a model for evaluating the ability of PNA probes for discriminating olive cultivars. Both unmodified and C2-modified PNAs bearing an arginine side-chain were used, the latter showing higher sequence specificity. DNA extracted from leaves of three different cultivars (Ogliarola leccese, Canino and Frantoio) could be easily discriminated using a microarray with unmodified PNA probes, whereas discrimination of DNA from oil samples was more challenging, and could be obtained only by using chiral PNA probes.

PMID:
22772038
PMCID:
PMC3429532
DOI:
10.4161/adna.20603
[Indexed for MEDLINE]
Free PMC Article
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