The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum

A J Evenden; M L Gilpin; C B Munn

doi:10.1016/0378-1097(90)90028-o

The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum

FEMS Microbiol Lett. 1990 Sep 1;59(1-2):31-4. doi: 10.1016/0378-1097(90)90028-o.

Authors

A J Evenden¹, M L Gilpin, C B Munn

Affiliation

¹ Department of Biological Sciences, Polytechnic South West, Plymouth, U.K.

PMID: 2276613
DOI: 10.1016/0378-1097(90)90028-o

Abstract

A gene encoding haemolytic activity from Renibacterium salmoninarum (strain PPD) was cloned into Escherichia coli using the cosmid vector pHC79, and subsequently subcloned on a 1.6 kbp SAlI fragment into pBR328. Southern blot hybridisation revealed that a homologous sequence is found in other strains of R. salmoninarum.

MeSH terms

Animals
Blotting, Southern
Cloning, Molecular
Cosmids
DNA, Bacterial / genetics
Escherichia coli / genetics
Genes, Bacterial
Gram-Positive Asporogenous Rods / genetics*
Gram-Positive Asporogenous Rods / pathogenicity
Hemolysin Proteins / genetics*
Hemolysis
Restriction Mapping
Salmonidae / microbiology
Sequence Homology, Nucleic Acid

Substances

DNA, Bacterial
Hemolysin Proteins