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Neurobiol Dis. 2012 Dec;48(3):391-8. doi: 10.1016/j.nbd.2012.06.018. Epub 2012 Jul 3.

Association of UBQLN1 mutation with Brown-Vialetto-Van Laere syndrome but not typical ALS.

Author information

1
Department of Neurology, University of Massachusetts Medical School, Worcester, MA 01655, USA. Paloma.Gonzalez-Perez@umassmed.edu

Abstract

Genetic variants in UBQLN1 gene have been linked to neurodegeneration and mutations in UBQLN2 have recently been identified as a rare cause of amyotrophic lateral sclerosis (ALS).

OBJECTIVE:

To test if genetic variants in UBQLN1 are involved in ALS.

METHODS:

102 and 94 unrelated patients with familial and sporadic forms of ALS were screened for UBQLN1 gene mutations. Single nucleotide variants were further screened in a larger set of sporadic ALS (SALS) patients and unrelated control subjects using high-throughput Taqman genotyping; variants were further assessed for novelty using the 1000Genomes and NHLBI databases. In vitro studies tested the effect of UBQLN1 variants on the ubiquitin-proteasome system (UPS).

RESULTS:

Only two UBQLN1 coding variants were detected in the familial and sporadic ALS DNA set; one, the missense mutation p.E54D, was identified in a single patient with atypical motor neuron disease consistent with Brown-Vialetto-Van Laere syndrome (BVVLS), for whom c20orf54 mutations had been excluded. Functional studies revealed that UBQLN1E54D protein forms cytosolic aggregates that contain mislocalized TDP-43 and impairs degradation of ubiquitinated proteins through the proteasome.

CONCLUSIONS:

Genetic variants in UBQLN1 are not commonly associated with ALS. A novel UBQLN1 mutation (E45D) detected in a patient with BVVLS altered nuclear TDP-43 localization in vitro, suggesting that UPS dysfunction may also underlie the pathogenesis of this condition.

PMID:
22766032
PMCID:
PMC4245016
DOI:
10.1016/j.nbd.2012.06.018
[Indexed for MEDLINE]
Free PMC Article

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