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J Biomed Nanotechnol. 2012 Jun;8(3):372-9.

A CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody.

Author information

1
Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, National Key Laboratory of Micro/Nano Fabrication Technology, Research Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Abstract

Herein we reported a CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody. The CdTe quantum dots were prepared, then were used to label Hepatitis B Virus surface antigen, and then were fabricated into lateral flow strips. The as-prepared lateral flow strips were used to test different concentration of anti-HBV surface antibodies. The CCD-based reader was designed and fabricated, the quantitative analysis software was compiled, and resultant CCD-based reader system was used for quantitative analysis of examined anti-HBs antibodies on the strips. Results showed that the quantum dots-labeled lateral flow strips could detect the anti-HBs antibody with the limitation concentration of 200 pg/mL, the CCD-based reader system could detect anti-HBs antibody with the sensitivity of 2 pg/mL. In conclusion, the prepared CCD-based reader combined quantum dots-labeled lateral flow strips can be used for quantitative detection of anti-HBs antibody in sera with the sensitivity of 2 pg/mL, and has great potential in applications such as ultrasensitive detection of HBV antigens or antibodies, and other tumor biomarkers in near future.

PMID:
22764406
[Indexed for MEDLINE]

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