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Anal Bioanal Chem. 2012 Sep;404(5):1327-30. doi: 10.1007/s00216-012-6218-x. Epub 2012 Jul 5.

High-speed X-ray microscopy by use of high-resolution zone plates and synchrotron radiation.

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National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China.


X-ray microscopy based on synchrotron radiation has become a fundamental tool in biology and life sciences to visualize the morphology of a specimen. These studies have particular requirements in terms of radiation damage and the image exposure time, which directly determines the total acquisition speed. To monitor and improve these key parameters, we present a novel X-ray microscopy method using a high-resolution zone plate as the objective and the matching condenser. Numerical simulations based on the scalar wave field theory validate the feasibility of the method and also indicate the performance of X-ray microscopy is optimized most with sub-10-nm-resolution zone plates. The proposed method is compatible with conventional X-ray microscopy techniques, such as computed tomography, and will find wide applications in time-resolved and/or dose-sensitive studies such as living cell imaging.

[Indexed for MEDLINE]

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