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Methods Mol Biol. 2012;895:167-96. doi: 10.1007/978-1-61779-927-3_13.

Wide-line NMR and protein hydration.

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1
Research Institute for Solid State Physics and Optics, Hungarian Academy of Sciences, Budapest, Hungary.

Abstract

In this chapter, the reader is introduced to the basics of wide-line NMR, with particular focus on the following: (1) basic theoretical and experimental NMR elements, necessary before switching the spectrometer and designing the experiment, (2) models/theories for the interpretation of measured data, (3) definition of wide-line NMR spectrometry, the description of the measurement and evaluation variants, useful hints for the novice, (4) advice on selecting the solvent, which is not a trivial task, (5) a note of warning that not all data are acceptable in spite of the statistical confidence. Finally, we wrap up the chapter with the results on two proteins (a globular and an intrinsically disordered).

PMID:
22760320
DOI:
10.1007/978-1-61779-927-3_13
[Indexed for MEDLINE]
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