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Viruses. 2012 May;4(5):847-77. doi: 10.3390/v4050847. Epub 2012 May 21.

Latest insights on adenovirus structure and assembly.

Author information

1
Department of Macromolecular Structures, Centro Nacional de Biotecnología (CNB-CSIC), Darwin 3, 28049 Madrid, Spain. carmen@cnb.csic.es

Erratum in

  • Viruses. 2012;4(12):3952.

Abstract

Adenovirus (AdV) capsid organization is considerably complex, not only because of its large size (~950 Å) and triangulation number (pseudo T = 25), but also because it contains four types of minor proteins in specialized locations modulating the quasi-equivalent icosahedral interactions. Up until 2009, only its major components (hexon, penton, and fiber) had separately been described in atomic detail. Their relationships within the virion, and the location of minor coat proteins, were inferred from combining the known crystal structures with increasingly more detailed cryo-electron microscopy (cryoEM) maps. There was no structural information on assembly intermediates. Later on that year, two reports described the structural differences between the mature and immature adenoviral particle, starting to shed light on the different stages of viral assembly, and giving further insights into the roles of core and minor coat proteins during morphogenesis [1,2]. Finally, in 2010, two papers describing the atomic resolution structure of the complete virion appeared [3,4]. These reports represent a veritable tour de force for two structural biology techniques: X-ray crystallography and cryoEM, as this is the largest macromolecular complex solved at high resolution by either of them. In particular, the cryoEM analysis provided an unprecedented clear picture of the complex protein networks shaping the icosahedral shell. Here I review these latest developments in the field of AdV structural studies.

KEYWORDS:

adenovirus; core proteins; cryo-electron microscopy; crystallography; maturation; minor coat proteins; structure

PMID:
22754652
PMCID:
PMC3386624
DOI:
10.3390/v4050847
[Indexed for MEDLINE]
Free PMC Article

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