Format

Send to

Choose Destination
See comment in PubMed Commons below
Int J Mol Sci. 2012;13(5):6073-88. doi: 10.3390/ijms13056073. Epub 2012 May 18.

Evaluation of antioxidant and free radical scavenging capacities of polyphenolics from pods of Caesalpinia pulcherrima.

Author information

1
Graduate Institute of Pharmacognosy, School of Pharmacy, Taipei Medical University, 250 Wuxing St., Taipei 11031, Taiwan; E-Mails: hsu0320@tmu.edu.tw (F.-L.H.); wjhuang@tmu.edu.tw (W.-J.H.); lmh@tmu.edu.tw (M.-H.L.); m303090001@tmu.edu.tw (H.-J.L.); wchou@tmu.edu.tw (W.-C.H.).

Abstract

Thirteen polyphenolics were isolated from fresh pods of Caesalpinia pulcherrima using various methods of column chromatography. The structures of these polyphenolics were elucidated as gallic acid (1), methyl gallate (2), 6-O-galloyl-d-glucoside (3), methyl 6-O-galloyl-β-d-glucoside (4), methyl 3,6-di-O-galloyl-α-d-glucopyranoside (5), gentisic acid 5-O-α-d-(6'-O-galloyl)glucopyranoside (6), guaiacylglycerol 4-O-β-d-(6'-O-galloyl)glucopyranoside (7), 3-methoxy-4-hydroxyphenol 1-O-β-d-(6'-O-galloyl) glucopyranoside (8), (+)-gallocatechin (9), (+)-catechin (10), (+)-gallocatechin 3-O-gallate (11), myricetin 3-rhamnoside (12), and ampelopsin (13). All isolated compounds were tested for their antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and peroxynitrite radicals scavenging assays. Among those compounds, 11, 12, and 2 exhibited the best DPPH-, hydroxyl-, and peroxynitrite radical-scavenging activities, respectively. Compound 7 is a new compound, and possesses better scavenging activities towards DPPH but has equivalent hydroxyl radical scavenging activity when compared to BHT. The paper is the first report on free radical scavenging properties of components of the fresh pods of Caesalpinia pulcherrima. The results obtained from the current study indicate that the free radical scavenging property of fresh pods of Caesalpinia pulcherrima may be one of the mechanisms by which this herbal medicine is effective in several free radical mediated diseases.

KEYWORDS:

1,1-diphenyl-2-picrylhydrazyl (DPPH) radical; butylated hydroxytoluene (BHT); dihydrorhodamine 123 (DHR 123); hydroxyl radical; peroxynitrite

PMID:
22754350
PMCID:
PMC3382783
DOI:
10.3390/ijms13056073
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
    Loading ...
    Support Center