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Afr J Tradit Complement Altern Med. 2011;8(5 Suppl):33-45. doi: 10.4314/ajtcam.v8i5S.13. Epub 2011 Jul 3.

Analysis of the extracts of Isatis tinctoria by new analytical approaches of HPLC, MS and NMR.

Author information

1
College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China. juezhou2006@yahoo.com.cn

Abstract

The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.

KEYWORDS:

HPLC; Isatis tinctoria; MS; NMR; alkaloids; indole glucosinolates (GLs)

PMID:
22754056
PMCID:
PMC3252730
DOI:
10.4314/ajtcam.v8i5S.13
[Indexed for MEDLINE]
Free PMC Article
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