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Int J Obes (Lond). 2013 Mar;37(3):399-403. doi: 10.1038/ijo.2012.105. Epub 2012 Jul 3.

Estimating energy expenditure in mice using an energy balance technique.

Author information

1
Division of Molecular Genetics and Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA.

Erratum in

  • Int J Obes (Lond).2013 Mar;37(3):473.

Abstract

OBJECTIVE:

To compare, in mice, the accuracy of estimates of energy expenditure (EE) using an energy balance technique (TEEbal: food energy intake and body composition change) vs indirect calorimetry (TEEIC).

SUBJECTS:

In 32 male C57BL/6J mice, EE was estimated using an energy balance (caloric intake minus change in body energy stores) method over a 37-day period. EE was also measured in the same animals by indirect calorimetry. These measures were compared.

RESULTS:

The two methods were highly correlated (r(2)=0.87: TEEbal=1.07*TEEIC-0.22, P<0.0001). By Bland-Altman analysis, TEEbal estimates were slightly higher (4.6±1.5%; P<0.05) than TEEIC estimates (Bias=0.55 kcal per 24 h).

CONCLUSION:

TEEbal can be performed in 'home cages' and provides an accurate integrated long-term measurement of EE while minimizing potentially confounding stress that may accompany the use of indirect calorimetry systems. The technique can also be used to assess long-term energy intake.

PMID:
22751256
PMCID:
PMC3697837
DOI:
10.1038/ijo.2012.105
[Indexed for MEDLINE]
Free PMC Article

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