Format

Send to

Choose Destination
See comment in PubMed Commons below
J Lipid Res. 2012 Sep;53(9):1823-31. doi: 10.1194/jlr.M022558. Epub 2012 Jun 29.

Global changes in phospholipids identified by MALDI MS in rats with focal cerebral ischemia.

Author information

1
Department of Molecular Biotechnology, WCU Program, Konkuk University, Seoul 143-701, Korea.

Abstract

Neuronal membrane phospholipids are highly affected by oxidative stress caused by ischemic injury. Thus, it is necessary to identify key lipid components that show changes during ischemia to develop an effective approach to prevent brain damage from ischemic injury. The recent development of MALDI imaging MS (MALDI IMS) makes it possible to identify phospholipids that change between damaged and normal regions directly from tissues. In this study, we conducted IMS on rat brains damaged by ischemic injury and detected various phospholipids that showed unique distributions between normal and damaged areas of the brain. Among them, we confirmed changes in phospholipids such as lysophosphatidylcholine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin by MALDI IMS followed by MS/MS analysis. These lipids were present in high concentrations in the brain and are important for maintenance of cellular structure as well as production of second messengers for cellular signal transduction. Our results emphasize the identification of phospholipid markers for ischemic injury and successfully identified several distinctly located phospholipids in ischemic brain tissue.

PMID:
22750654
PMCID:
PMC3413223
DOI:
10.1194/jlr.M022558
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center