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Toxicon. 2012 Oct;60(5):874-7. doi: 10.1016/j.toxicon.2012.06.014. Epub 2012 Jun 29.

Resolution of two overlapping neutralizing B cell epitopes within a solvent exposed, immunodominant α-helix in ricin toxin's enzymatic subunit.

Author information

1
Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.

Abstract

Residues Y₉₁-T₁₁₆ of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs), PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q₉₈, E₁₀₂, T₁₀₅, and H₁₀₆ are central to mAb recognition.

PMID:
22750533
PMCID:
PMC3461947
DOI:
10.1016/j.toxicon.2012.06.014
[Indexed for MEDLINE]
Free PMC Article

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