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J Neurosci Methods. 2012 Aug 15;209(2):398-402. doi: 10.1016/j.jneumeth.2012.06.018. Epub 2012 Jun 28.

The comparison of three methods of drawing cerebrospinal fluid in rabbit.

Author information

1
Department of Neuroscience, Institute for Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, China.

Abstract

Component analysis of cerebrospinal fluid (CSF) is frequently required to probe the cause of disease, pathologic mechanisms and effective drugs in the experimental study of central nervous system. An ideal way of drawing CFS is very important for the successful analysis. However, pros and cons of types of CSF drawing have never been analyzed and compared. The purpose of this study was to choose an ideal method of obtaining CSF in rabbits by comparing the three usual ones: skull drilling, lumbar puncture and atlanto-occipital membrane puncture. In the study, the rabbits were randomly divided into three groups: skull drilling group, lumbar puncture group and atlanto-occipital membrane puncture group, and our modified puncture needles were used. The rates of success, operating time and repeatability, etc. of the three methods were compared. The results suggest that the atlanto-occipital membrane puncture method is the best. The method was the simplest, fastest, and most repeatable of the three, furthermore, with this method, the most CSF was drawn and achieved the highest success rate.

PMID:
22750266
DOI:
10.1016/j.jneumeth.2012.06.018
[Indexed for MEDLINE]

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