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Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):11770-5. doi: 10.1073/pnas.1203405109. Epub 2012 Jun 28.

LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection.

Author information

1
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Abstract

Recognition of viral nucleic acids by pattern recognition receptors initiates type I IFN induction and innate antiviral immune response. Here we show that LSm14A, a member of the LSm family involved in RNA processing in the processing bodies, binds to synthetic or viral RNA and DNA and mediates IRF3 activation and IFN-β induction. Knockdown of LSm14A inhibits cytosolic RNA- and DNA-trigger type I IFN production and cellular antiviral response. Moreover, LSm14A is essential for early-phase induction of IFN-β after either RNA or DNA virus infection. We further found that LSm14A-mediated IFN-β induction requires RIG-I-VISA or MITA after RNA or DNA virus infection, respectively, and viral infection causes translocation of LSm14A to peroxisomes, where RIG-I, VISA, and MITA are located. These findings suggest that LSm14A is a sensor for both viral RNA and DNA and plays an important role in initiating IFN-β induction in the early phase of viral infection.

PMID:
22745163
PMCID:
PMC3406844
DOI:
10.1073/pnas.1203405109
[Indexed for MEDLINE]
Free PMC Article

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