Format

Send to

Choose Destination
See comment in PubMed Commons below
Dev Biol. 2012 Sep 1;369(1):124-32. doi: 10.1016/j.ydbio.2012.06.013. Epub 2012 Jun 26.

The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.

Author information

1
Department of Biology, Emory University, Atlanta, GA 30322, United States.

Abstract

Boundary Element Associated Factor-32 (BEAF-32) is an insulator protein predominantly found near gene promoters and thought to play a role in gene expression. We find that mutations in BEAF-32 are lethal, show loss of epithelial morphology in imaginal discs and cause neoplastic growth defects. To investigate the molecular mechanisms underlying this phenotype, we carried out a genome-wide analysis of BEAF-32 localization in wing imaginal disc cells. Mutation of BEAF-32 results in miss-regulation of 3850 genes by at least 1.5-fold, 794 of which are bound by this protein in wing imaginal cells. Up-regulated genes encode proteins involved in cell polarity, cell proliferation and cell differentiation. Among the down-regulated genes are those encoding components of the wingless pathway, which is required for cell differentiation. Miss-regulation of these genes explains the unregulated cell growth and neoplastic phenotypes observed in imaginal tissues of BEAF-32 mutants.

PMID:
22743648
PMCID:
PMC3572785
DOI:
10.1016/j.ydbio.2012.06.013
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center