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Biomed Opt Express. 2012 Jun 1;3(6):1241-55. doi: 10.1364/BOE.3.001241. Epub 2012 May 3.

Depth-resolved cellular microrheology using HiLo microscopy.

Abstract

It is increasingly important to measure cell mechanical properties in three-dimensional environments. Particle tracking microrheology (PTM) can measure cellular viscoelastic properties; however, out-of-plane data can introduce artifacts into these measurements. We developed a technique that employs HiLo microscopy to reduce out-of-plane contributions. This method eliminated signals from 90% of probes 0.5 μm or further from the focal plane, while retaining all in-plane probes. We used this technique to characterize live-cell bilayers and found that there were significant, frequency-dependent changes to the extracted cell moduli when compared to conventional analysis. Our results indicate that removal of out-of-plane information is vital for accurate assessments of cell mechanical properties.

KEYWORDS:

(170.1530) Cell analysis; (180.0180) Microscopy

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