Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Commun. 2012 Jun 26;3:928. doi: 10.1038/ncomms1925.

Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded light.

Author information

1
Bioengineering and Electrical Engineering, California Institute of Technology, 1200 E California Boulevard, Pasadena, California 91125, USA. yingmin@caltech.edu

Abstract

Fluorescence imaging is one of the most important research tools in biomedical sciences. However, scattering of light severely impedes imaging of thick biological samples beyond the ballistic regime. Here we directly show focusing and high-resolution fluorescence imaging deep inside biological tissues by digitally time-reversing ultrasound-tagged light with high optical gain (~5×10(5)). We confirm the presence of a time-reversed optical focus along with a diffuse background-a corollary of partial phase conjugation-and develop an approach for dynamic background cancellation. To illustrate the potential of our method, we image complex fluorescent objects and tumour microtissues at an unprecedented depth of 2.5 mm in biological tissues at a lateral resolution of 36 μm×52 μm and an axial resolution of 657 μm. Our results set the stage for a range of deep-tissue imaging applications in biomedical research and medical diagnostics.

PMID:
22735456
PMCID:
PMC3621452
DOI:
10.1038/ncomms1925
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center