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J Am Chem Soc. 2012 Jul 18;134(28):11338-41. doi: 10.1021/ja303226x. Epub 2012 Jul 2.

Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering.

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1
Department of Chemistry, Princeton University, 325 Frick Chemistry Laboratory, Princeton, New Jersey 08544, USA.

Abstract

We describe the first systematic study of a family of inteins, the split DnaE inteins from cyanobacteria. By measuring in vivo splicing efficiencies and in vitro kinetics, we demonstrate that several inteins can catalyze protein trans-splicing in tens of seconds rather than hours, as is commonly observed for this autoprocessing protein family. Furthermore, we show that when artificially fused, these inteins can be used for rapid generation of protein α-thioesters for expressed protein ligation. This comprehensive survey of split inteins provides indispensable information for the development and improvement of intein-based tools for chemical biology.

PMID:
22734434
PMCID:
PMC3535263
DOI:
10.1021/ja303226x
[Indexed for MEDLINE]
Free PMC Article
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