Format

Send to

Choose Destination
See comment in PubMed Commons below
Chembiochem. 2012 Jul 23;13(11):1559-63. doi: 10.1002/cbic.201200256. Epub 2012 Jun 22.

Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.

Author information

1
Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK.

Abstract

Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.

PMID:
22730246
DOI:
10.1002/cbic.201200256
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center